A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping
Abstract Background Puccinia striiformis f.sp.tritici (PST), an obligate fungal pathogen causing wheat yellow/stripe rust, a serious disease, has been used to understand the evolution of crop pathogen using molecular markers.However, numerous questions regarding its evolutionary history and recent migration routes still remains to be addressed, whi